Newcastle disease vaccination regimen comprising both Lentogenic and Mesogenic strains is more effective than Lentogenic strain only

Plaque assay for avirulent (lentogenic) strains of Newcastle disease virus.

Avirulent (lentogenic) strains of Newcastle disease virus form plaques on chicken embryo lung monolayers in 48 to 72 h.

Recombinant Immunomodulating Lentogenic or Mesogenic Oncolytic Newcastle Disease Virus for Treatment of Pancreatic Adenocarcinoma

Oncolytic Newcastle disease virus (NDV) might be a promising new therapeutic agent for the treatment of pancreatic cancer. We evaluated recombinant NDVs (rNDVs) expressing interferon (rNDV-hIFNβ-F₀) or an IFN antagonistic protein (rNDV-NS1-F₀), as well as rNDV with increased virulence (rNDV-F₃aa) for oncolytic efficacy in human pancreatic adenocarcinoma cells. Expression of additional proteins ...

Generation of recombinant lentogenic Newcastle disease virus from cDNA.

Recombinant lentogenic Newcastle disease virus (NDV) of the vaccine strain Clone-30 was reproducibly generated after simultaneous expression of antigenome-sense NDV RNA and NDV nucleoprotein, phosphoprotein and RNA-dependent RNA polymerase from plasmids transfected into cells stably expressing T7 RNA polymerase. For this purpose, the genome of Clone-30, comprising 15186 nt, was cloned and seque...

Studies on the Immunogenic Properties of Three Lentogenic Strains of Newcastle Disease Virus

The HI and SN titers produced by V2 (Laso ta Vaccine, chick-embryo origin) were more than VI (TCND) and V3 (Lasota tissue culture origin) vaccines. High percentage (96,%, 93,% & 90,%) of the three subgroup hens, when vaccinated with VI, V2 and V3 respectively, were found resistant to contact exposure challenge with 2 x 10 6 ELD 50 of virulent NDV Zabol strain. The egg production dropped slightl...

Studies on the immunogenic properties of three lentogenic strains of newcastle disease virus